| 1. Prepare the sample |  |
| Prepare the dsDNA obtained by customer’s PCR assay, or an RNA sample extracted from a biological sample, and then purify it.
Return MultiNA proprietary reagents (separation buffer, markers) and fluorescent dye reagent to room temperature.
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| 2. Create the analysis schedule |  |
| Start the PC-based MultiNA instrument control software. Register an analysis schedule on the MultiNA instrument control software according to the samples. The schedule can be imported and created in CSV format. |
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| 3. Prepare the MultiNA reagents |  |
Prepare the following reagents according to the analysis schedule.
Prepare:
- the separation buffer by diluting the fluorescent dye and stirring it.
- the specified quantity of marker according to the mixture mode.
- the required quantity of ladder for generating the size calibration curve
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| 4. Load the instrument |  |
| If analysis is being conducted for the first time or if the microchip is being replaced, set the microchip(s) in the instrument. Set the ladder, sample, separation buffer and marker at the position registered in the analysis schedule. Replenish the wash water and empty the waste liquid bottle. |
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| 5. Start analysis |  |
| Close the top cover, and press the Start button displayed in the MultiNA instrument control software. |
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| 6. Check the analysis results |  |
| Press the View Data File button in the MultiNA instrument control software. The Viewer opens to allow viewing of the results for samples already analyzed.
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